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BRIEF RESEARCH REPORT article
Front. Nephrol.
Sec. Glomerular disease
Volume 4 - 2024 |
doi: 10.3389/fneph.2024.1471078
Increased levels of antibodies to synaptopodin and annexin 1 in patients with primary podocytopathies
Provisionally accepted- 1 I.M. Sechenov First Moscow State Medical University, Moscow, Russia
- 2 Lomonosov Moscow State University, Moscow, Moscow, Russia
- 3 P.Herzen Moscow Oncology Research Institute (MORI), Moscow, Moscow Oblast, Russia
- 4 Skolkovo Institute of Science and Technology, Moscow, Moscow Oblast, Russia
Circulating anti-podocyte antibodies have been proposed as potential factors contributing to increased permeability in primary podocytopathies, such as Minimal Change Disease (MCD) and Focal Segmental Glomerulosclerosis (FSGS). The aim of the study was to to assess the levels of antibodies targeting synaptopodin and annexin 1 in the blood serum of patients diagnosed with nephrotic syndrome, with the aim of evaluating their potential utility in diagnosing primary podocytopathies. Methods: The study included a total of 72 patients diagnosed with nephrotic syndrome, alongside 21 healthy subjects for comparison. Among the patients, 38 were diagnosed with FSGS, 12 with MCD, and 22 with MN. The levels of anti-synaptopodin and anti-annexin-1 antibodies were quantified using ELISA.Results: The levels of antibodies to annexin 1 and anti-synaptopodin in the blood were found to be higher in patients diagnosed with MCD and FSGS compared to those with MN and healthy individuals. A model incorporating both antibodies demonstrated higher sensitivity (80.9%) and specificity (81.3%) with an AUC of 0.859 (95% CI 0.760-0.957). Notably, serum levels of annexin 1 and anti-synaptopodin antibodies did not predict the response to prednisolone and/or CNI therapy.Discussion. Levels of antibodies targeting synaptopodin and annexin 1 were notably elevated in patients diagnosed with MCD and FSGS compared to those with MN and healthy controls. A panel comprising both antibodies demonstrated moderate to high sensitivity and specificity for diagnosis MCD or FSGS.
Keywords: minimal change disease, focal segmental glomerulosclerosis, Podocytopathy, Annexin 1, Synaptopodin
Received: 26 Jul 2024; Accepted: 07 Oct 2024.
Copyright: © 2024 Chebotareva, Kharionovskaya, Birukova, Vinogradov, Sergeeva, Alentov, Kononikhin, Nikolaev and Moiseev. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Natalia Chebotareva, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
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