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METHODS article

Front. Nat. Prod.
Sec. Isolation and Purification
Volume 3 - 2024 | doi: 10.3389/fntpr.2024.1532543
This article is part of the Research Topic Celebrating Advances and Future Directions in Natural Products Research View all 4 articles

MATRIX: A Platform for Cultivation Profiling and Bioactive Discovery in Natural Products Research

Provisionally accepted
Zeinab G Khalil Zeinab G Khalil *Thulasi Sritharan Thulasi Sritharan Angela Aguslyarti Salim Angela Aguslyarti Salim Rob Capon Rob Capon *
  • The University of Queensland, Brisbane, Australia

The final, formatted version of the article will be published soon.

    The MATRIX protocol presents a novel miniaturized 24-well plate approach for microbial cultivation profiling, enabling the efficient and parallel assessment of microbial metabolite production. This method integrates a media MATRIX within a microbioreactor that facilitate the creation of diverse growth conditions for microbial cultivation. The 24-well format supports the simultaneous study of multiple microbial strains and conditions, allowing the discovery of novel bioactive compounds. By employing a variety of media compositions and cultivations, MATRIX enhances the understanding of microbial metabolic pathways and interactions. This protocol provides a robust platform for natural product discovery, microbial ecology studies, and drug development, showcasing its potential to uncover new natural products and other valuable bioactives. The MATRIX approach offers a resource-efficient platform for microbial biodiscovery, contributing significantly to natural product research and drug development.

    Keywords: Matrix methodology, Microbial cultivation profiling, natural product discovery, bioactive compounds, Biosynthetic gene clusters, High-Throughput Screening, secondary metabolites, GNPS molecular networking

    Received: 22 Nov 2024; Accepted: 18 Dec 2024.

    Copyright: © 2024 Khalil, Sritharan, Salim and Capon. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Zeinab G Khalil, The University of Queensland, Brisbane, Australia
    Rob Capon, The University of Queensland, Brisbane, Australia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.