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ORIGINAL RESEARCH article

Front. Nat. Prod.
Sec. Isolation and Purification
Volume 3 - 2024 | doi: 10.3389/fntpr.2024.1322933
This article is part of the Research Topic The Potential Role of Natural Products in Sustainable Agri-food Systems View all articles

Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from the fruit of Rosa roxburghii Tratt

Provisionally accepted
Yao Wen Yao Wen *Geping Chen Geping Chen
  • Guangdong Pharmaceutical University, Guangzhou, China

The final, formatted version of the article will be published soon.

    The polysaccharide was extracted by ultrasonic-assisted method with deep eutectic solvent from Rosa roxburghii Tratt. (RRT). The single-factor experiment was carried out first, and then the response surface experiment was designed to obtain the optimal process conditions. The optimal extraction conditions were the molar ratio of choline chloride to oxalate of 1:1, the water content of DES of 27%, the solid-liquid ratio of 1:52 g/mL, time of 99 min, and the power of 240 W, with the extraction yield of 3.397%. The yield of RRTPs was 2.693% and 2.521% by water immersion and ultrasonic water extraction, respectively, indicating that the DES extraction method (3.397%) is superior to these two methods, and the optimization effect is good. After purification by the DEAE column and Sephadex G-75 column, the biological activities of the extracted polysaccharides were tested. At a concentration of 6.4 mg/mL, RRTPs-1 inhibited α-glucosidase by 26%. The IC50 value of RRTPs-1 against α-amylase was 2.94 mg/mL. At 3.2 mg/mL, RRTPs-1 inhibited DPPH· by 22.36%. The IC50 value of RRTPs-1 on ABTS·+ scavenging was 0.055 mg/mL. The analysis of monosaccharide composition shows that it contains glucose, rhamnose, xylose, arabinose, mannose, galactose, galacturonic acid, and glucuronic acid, with the molar ratio of 1.00: 14.14: 4.28: 5.63: 15.47: 64.50: 5.03: 41.43. In this paper, the process of extracting RRT polysaccharide (RRTP) with deep eutectic solvent was studied and optimized. The activity study showed that RRTP has certain hypoglycemic and antioxidant activities. Therefore, this study can provide an experimental basis for the establishment of an industrialized production process of polysaccharides and the study of their biological activities.

    Keywords: Rosa roxburghii Tratt. polysaccharide, Deep eutectic solvent, Response surface optimization, Monosaccharide composition, Biological activities

    Received: 17 Oct 2023; Accepted: 16 Apr 2024.

    Copyright: © 2024 Wen and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yao Wen, Guangdong Pharmaceutical University, Guangzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.