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ORIGINAL RESEARCH article
Front. Nanotechnol.
Sec. Biomedical Nanotechnology
Volume 6 - 2024 |
doi: 10.3389/fnano.2024.1465888
This article is part of the Research Topic Nanocomposites in medical applications: Bridging the gap between materials science and medicine View all articles
Formation of self-nanoparticles and immune effect on tumor after injection of ferric chloride with H2O2 under magnetic field therapy
Provisionally accepted- 1 Beijing Cancer Hospital, Beijing, China
- 2 Jinan Baofa Cancer Hospital, Jinan, Shandong Province, China
Objective: Nanotechnology offers many advantages in various fields of cancer therapy. Here, a new method of self-forming nanoparticles (Self-Nano) is described using ferric chloride (FeCl₃) and hydrogen peroxide (H2O2) to form the self-nanoparticles in an in vivo tumor. The treatment effect is evaluated.Method: A solution of 3% FeCl3 (0.5ml) and 1.8% H2O2 (1.0ml) was injected into the tumor. At various time points post-injection, tumors were collected, and sections were prepared for an electron microscope to evaluate the size of self-nano particles. Single-cell RNA sequencing (scRNA-seq) was used to analyze the immune changes and their effect their effects on tumor growth.Result: The formation of self-nano in vitro was observed and confirmed, with particles averaging 421 nm in size for the FeCl3 +H2O2 solution. Over time points ranging from 1 to 14 days, the formed self-nano remained stable at a regular size of 421±8 nm. The self-nano, primarily consisting of iron, induced ferroptosis, under the influence of an external magnetic field leading to tumor growth control through iron-induced cell death and immune reactions. These self-nanoparticles also showed stronger enrichment of pathways related to CD8 + T effect cells (Teff), T cell activation, and regulation of T cell proliferation.The FeCl3+H2O2 solution can form Fe₂O₃-based self-nanoparticles within tumors through H2O2-incubated oxidation of FeCI3. The self-nano remains effective for over 14 days, inducing ferroptosis and upregulating immune cells under magnetic field treatment. This approach offers a novel approach for cancer treatment that can be combined with other modalities.
Keywords: Nanoparticles, Self-Nano , Hydrogen peroxide (H2O2) , Ferroptosis, Ferric chloride (FeCl3), Iron(III) oxide (Fe₂O₃), tumor therapy, Magnetic field treatment
Received: 17 Jul 2024; Accepted: 26 Aug 2024.
Copyright: © 2024 Yu, Han, Zhang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Baofa Yu, Beijing Cancer Hospital, Beijing, China
Yan Han, Jinan Baofa Cancer Hospital, Jinan, Shandong Province, China
Jian Zhang, Jinan Baofa Cancer Hospital, Jinan, Shandong Province, China
Dong Chen, Jinan Baofa Cancer Hospital, Jinan, Shandong Province, China
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