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ORIGINAL RESEARCH article

Front. Nanotechnol.
Sec. Biomedical Nanotechnology
Volume 6 - 2024 | doi: 10.3389/fnano.2024.1392694
This article is part of the Research Topic Nanotechnologies as a Novel Strategy for Enzyme Protection and Delivery View all articles

Effect of Chiral Silver Nanoparticles on Prolyl-oligopeptidase Binding and Activity

Provisionally accepted
Carin C. Batista Carin C. Batista 1Victor H. Toledo Victor H. Toledo 1Marcos P. Ramos Marcos P. Ramos 2Victor Oliveira Victor Oliveira 2Javier Acuña Javier Acuña 1Marcelo Y. Icimoto Marcelo Y. Icimoto 2*ISELI L. NANTES ISELI L. NANTES 3*
  • 1 Federal University of ABC, Santo André, São Paulo, Brazil
  • 2 Paulista School of Medicine, Federal University of São Paulo, São Paulo, São Paulo, Brazil
  • 3 Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, Brazil

The final, formatted version of the article will be published soon.

    Silver nanoparticles have a diversity of applications both in biological and technological areas. More recently, studies conducted in the Nano/Bio interface have demonstrated that chiral nanocrystals grew in chiral templates, and nanostructures functionalized with chiral molecules present specific properties. These properties apply to advanced materials, energy, medicine, and pharmacology. The present study synthesized silver nanoparticles on silver seeds using D-and L-histidine as templates and borohydride as a reducing agent. The nanoparticles were characterized by UV-visible spectroscopy and presented surface plasmon resonance (SPR) bands around 415 nm. CD spectra showed signals in the region of the SPR band, indicating the growth of nanocrystals with chiral distortion. Synthesized silver nanoparticles were also characterized by high-resolution transmission electronic microscopy (HRTEM), which evidenced the presence of histidine corona. The silver nanoparticles were functionalized with prolyl-oligopeptidase (POP), a proline-specific endopeptidase expressed in the brain. This enzyme cleaves neuroactive peptides involved in memory, learning, and neurodegeneration. The enzyme POP was expressed with a His-tag to provide competitive binding affinity to silver nanoparticles covered by D-and L-histidine. Considering the biological importance, POP was chosen as a model for studying the functionalization of chiral silver nanoparticles regarding the chiral discrimination for binding affinity and stabilization.

    Keywords: chiral silver nanoparticles, Histidine, prolyl-oligopeptidase, binding affinity, Circular Dichroism, HRTEM

    Received: 27 Feb 2024; Accepted: 09 Jul 2024.

    Copyright: © 2024 Batista, Toledo, Ramos, Oliveira, Acuña, Icimoto and NANTES. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Marcelo Y. Icimoto, Paulista School of Medicine, Federal University of São Paulo, São Paulo, 04023-062, São Paulo, Brazil
    ISELI L. NANTES, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, Brazil

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.