AUTHOR=Paxinou Alexandra , Marcello Elena , Vecchiato Vittoria , Erman Lara , Wright Edward , Noble Brendon , McCormick Adele , Basnett Pooja 

TITLE=Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles

JOURNAL=Frontiers in Nanotechnology

VOLUME=5

YEAR=2023

URL=https://www.frontiersin.org/journals/nanotechnology/articles/10.3389/fnano.2023.1243056

DOI=10.3389/fnano.2023.1243056

ISSN=2673-3013

ABSTRACT=<p>Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as <italic>Pseudomonas mendocina</italic> CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against <italic>Staphylococcus aureus</italic> 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.</p>