Erratum: Uncovering novel KCC2 regulatory motifs through a comprehensive transposon-based mutant library

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1. Uncovering novel KCC2 regulatory motifs through a comprehensive transposon-based mutant library

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An Erratum on
Uncovering novel KCC2 regulatory motifs through a comprehensive transposon-based mutant library

by Uvarov, P., Fudo, S., Karakus, C., Golubtsov, A., Rotondo, F., Sukhanova, T., Soni, S., Di Scala, C., Kajander, T., Rivera, C., and Ludwig, A. (2025). Front. Mol. Neurosci. 17:1505722. doi: 10.3389/fnmol.2024.1505722

Due to a production error, there was a mistake in the published legend of Figure 4, resulting in the incorrect use and duplication of the legend for Figure 3. The corrected Figure 4 and its legend appear below.

Figure 4

Figure 4. Glycosylation patterns of the KCC2-CTD mutants. (A) Top panel: Western blot analysis of KCC2 polypeptides in the total protein lysates of HEK293 cells expressing KCC2-CTD mutants, using a KCC2 antibody recognizing the C-terminal epitope. Two bands around 130-kDa corresponding to the putative glycosylated forms of monomeric KCC2 are observed. Bottom panel: To ensure equal amounts of total proteins loaded on SDS–PAGE, blots were analyzed with the antibody recognizing α-Tubulin. (B) PNGase F treatment removes all N-linked oligosaccharides from the KCC2 polypeptides, thus shifting the KCC2 bands to their predicted unglycosylated molecular weight of 123.6-kDa. (C) Endo H treatment shifts down the 125-kDa band corresponding to the A7, A8, and B1 KCC2 mutants, which contain mainly ER-added high-mannose glycans but leaves intact the 140-kDa KCC2 band corresponding to the B8 and C1 mutants, which contain mainly Golgi-added complex glycans.

The publisher apologizes for this mistake.

The original article has been updated.