The serum proteomic profile in patients with migraine

Yating Han ¹ Yuan Wang ² Xiajuan Zou ^3* Huailian Guo ^1,4*

• ¹ Department of Neurology, Peking University People’s Hospital, Beijing, Beijing Municipality, China
• ² College of Chemistry and Molecular Engineering, Peking University, Beijing, China
• ³ Medical and Healthy Analysis Center, Peking University, Beijing, China
• ⁴ Key Laboratory for Neuroscience, Ministry of Education, National Health and Family Planning Commission, Peking University, Beijing, China

Background: Migraine is a paroxysmal headache disorder, which seriously affected the patients' quality of life. However, the pathogenesis of migraine isn't clear yet. Proteomics is as an emerging technology to study small molecules and protein components in biological systems. The aim of the study was to analyze the serum proteome of migraine patients and healthy controls and identify differentially expressed proteins, which could provide reference for the study of biomarkers and pathophysiological mechanism of migraine. Methods: Fasting venous blood was collected and serum was separated. Liquid chromatography-mass spectrometry (LC-MS) was used to detect the proteome of the two groups, and Maxquant was used to analyze the protein profile and identify the differentially expressed proteins. Results: Twenty-seven migraine patients and 20 healthy people matching the age and sex ratio of the migraine group were collected. A total of 27 differentially expressed proteins were identified between migraine and control groups, which were mainly related to immune response, inflammation, glycolysis, lipid metabolism, neurotrophy and development, and so on. Subgroup analysis also identified several differentially expressed proteins between migraine with aura (MA) and migraine without aura (MO) groups, ictal and interictal period. And the signal pathways that may be related to migraine include glycolysis/gluconeogenesis pathway and hypoxia inducible factors-1 (HIF-1) signal pathway. Differentially expressed proteins are mainly distributed in the extracellular area. Related biological processes include complement activation, immunoglobulin receptor binding, phagocytosis, and so on. Discussion: The research screened out several differentially expressed proteins of migraine patients, which may be potential biomarkers, but it still needs verification in further study with larger sample size. Various proteins related to inflammation, immune response and energy metabolism are differentially expressed between the migraine group and the control 删除了: There were 8 38 删除了: related to oxygen transport, immunity, and so on. 39 And 4 differentially expressed proteins were identified 40 between … 41 删除了: of migraine groups, related to inflammation and 42 immunity 43 删除了: GO and KEGG analysis indicate that