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ORIGINAL RESEARCH article

Front. Mol. Neurosci.
Sec. Molecular Signalling and Pathways
Volume 17 - 2024 | doi: 10.3389/fnmol.2024.1518737

Impact of Modulating Leptin Sensitivity on the Transcriptomic Profile of Adult-Derived Hypothalamic Mouse Neurons

Provisionally accepted
Ewa Ocłoń Ewa Ocłoń 1*Artur Gurgul Artur Gurgul 2Igor Jasielczuk Igor Jasielczuk 2Tomasz Szmatola Tomasz Szmatola 2Miroslaw Kucharski Miroslaw Kucharski 3Joanna Zubel-Lojek Joanna Zubel-Lojek 3Dorota Zieba-Przybylska Dorota Zieba-Przybylska 4*
  • 1 Laboratory of Recombinant Proteins Production, University of Agriculture in Krakow, Krakow, Poland
  • 2 Department Of Basic Sciences, University of Agriculture in Krakow, Krakow, Poland
  • 3 Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Krakow, Poland
  • 4 Department of Animal Biotechnology, University of Agriculture in Krakow, Krakow, Poland

The final, formatted version of the article will be published soon.

    The modulation of leptin sensitivity in hypothalamic neurons plays a crucial role in metabolic regulation and the development of obesity. Three distinct approaches, exposure to leptin (LEPA), administration of leptin antagonist (LANTA), and treatment with palmitate (PA), were explored in this study to assess their effects on adult-derived mHypoA-2/12 neurons and the resulting transcriptomic signatures. To this end, 3' mRNA-Seq transcriptome analysis was employed, unexpectedly revealing downregulation of key genes associated with the NOD-like receptor signaling pathway (Irf9,

    Keywords: Leptin, hypothalamic neurons, Palmitic Acid, leptin resistance, Inflammation, RNAseq and Traf5), the C-type lectin receptor signaling pathway (Nfkb2, IRF9

    Received: 28 Oct 2024; Accepted: 20 Dec 2024.

    Copyright: © 2024 Ocłoń, Gurgul, Jasielczuk, Szmatola, Kucharski, Zubel-Lojek and Zieba-Przybylska. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ewa Ocłoń, Laboratory of Recombinant Proteins Production, University of Agriculture in Krakow, Krakow, Poland
    Dorota Zieba-Przybylska, Department of Animal Biotechnology, University of Agriculture in Krakow, Krakow, Poland

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.