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BRIEF RESEARCH REPORT article

Front. Mol. Neurosci.
Sec. Molecular Signalling and Pathways
Volume 17 - 2024 | doi: 10.3389/fnmol.2024.1444629

Calcium-dependent activator protein for secretion 2 is involved in dopamine release in mouse midbrain neurons

Provisionally accepted
Hirotoshi Iguchi Hirotoshi Iguchi 1Takumi Katsuzawa Takumi Katsuzawa 2Chihiro Saruta Chihiro Saruta 3Tetsushi Sadakata Tetsushi Sadakata 4Shota Kobayashi Shota Kobayashi 1Yumi Sato Yumi Sato 5Akira Sato Akira Sato 1Yoshitake Sano Yoshitake Sano 1So Maezawa So Maezawa 1Yo Shinoda Yo Shinoda 2*Teiichi Furuichi Teiichi Furuichi 1*
  • 1 Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan
  • 2 Tokyo University of Pharmacy and Life Sciences, Hachioji, Tōkyō, Japan
  • 3 RIKEN Brain Science Institute (BSI), Wako, Saitama, Japan
  • 4 Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan
  • 5 School of Medicine, Fukushima Medical University, Fukushima, Fukushima, Japan

The final, formatted version of the article will be published soon.

    The Ca 2+ -dependent activator protein for secretion (CAPS/CADPS) family protein facilitates catecholamine release through the dense-core vesicle exocytosis in model neuroendocrine cell lines. However, it remains unclear if it induces dopamine release in the central neurons. This study aimed to examine the expression and function of CADPS2, one of the two CADPS paralogs, in dopamine neurons of the mouse midbrain. This study shows that CADPS2 was expressed in tyrosine hydroxylase and the vesicular monoamine transporter 2 (VMAT2)-positive dopaminergic neurons of the midbrain samples and primary mesencephalic cell cultures. Subcellular fractions rich in dopamine were collected using immunoaffinity for CADPS2 from midbrain protein extracts. Cell imaging using fluorescent false neurotransmitter FFN511 as a substrate for VMAT2 showed decreased activity-dependent dopamine release in Cadps2-deficient cultures, compared to that in wild-type cultures. These results suggest that CADPS2 is involved in dopamine release from the central neurons, indicating its involvement in the central dopamine pathway.

    Keywords: Cadps2, Dopamine, Exocytosis, midbrain, tyrosine hydroxylase Article types: Brief Research Report

    Received: 06 Jun 2024; Accepted: 28 Jun 2024.

    Copyright: © 2024 Iguchi, Katsuzawa, Saruta, Sadakata, Kobayashi, Sato, Sato, Sano, Maezawa, Shinoda and Furuichi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yo Shinoda, Tokyo University of Pharmacy and Life Sciences, Hachioji, １４３２−1, Tōkyō, Japan
    Teiichi Furuichi, Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, Noda, Japan

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