AUTHOR=Smith Eliot T. , Sun Peng , Yu Shengyang Kevin , Raible David W. , Nicolson Teresa 

TITLE=Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish

JOURNAL=Frontiers in Molecular Neuroscience

VOLUME=16

YEAR=2023

URL=https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2023.1274822

DOI=10.3389/fnmol.2023.1274822

ISSN=1662-5099

ABSTRACT=<p>Ciliated sensory cells such as photo- and olfactory receptors employ multiple types of opsins or hundreds of unique olfactory G-protein coupled receptors to respond to various wavelengths of light or odorants. With respect to hearing and balance, the mechanotransduction machinery involves fewer variants; however, emerging evidence suggests that specialization occurs at the molecular level. To address how the mechanotransduction complex varies in the inner ear, we characterized the expression of paralogous genes that encode components required for mechanotransduction in zebrafish hair cells using RNA-FISH and bioinformatic analysis. Our data indicate striking zonal differences in the expression of two components of the mechanotransduction complex which are known to physically interact, the <italic>transmembrane channel-like 1</italic> and <italic>2</italic> (<italic>tmc1/2</italic>) family members and the <italic>calcium and integrin binding 2</italic> and <italic>3</italic> (<italic>cib2/3</italic>) paralogues. <italic>tmc1</italic>, <italic>tmc2b</italic>, and <italic>cib3</italic> are largely expressed in peripheral or extrastriolar hair cells, whereas <italic>tmc2a</italic> and <italic>cib2</italic> are enriched in central or striolar hair cells. In addition, a gene implicated in deaf-blindness, <italic>ush1c</italic>, is highly enriched in a subset of extrastriolar hair cells. These results indicate that specific combinations of these components may optimize responses to mechanical stimuli in subtypes of sensory receptors within the inner ear.</p>