AUTHOR=Chew Leon H. , Mercier Eloi , Rogalski Jason C. , Pippard Sara , Knock Erin 

TITLE=Methods to extract and analyze fluid from human pluripotent stem cell-derived choroid plexus organoids

JOURNAL=Frontiers in Molecular Neuroscience

VOLUME=16

YEAR=2024

URL=https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2023.1243499

DOI=10.3389/fnmol.2023.1243499

ISSN=1662-5099

ABSTRACT=<p>The choroid plexus (ChP) is a highly vascularized tissue lining the ventricular space of the brain. The ChP generates cerebrospinal fluid (CSF) and forms a protective barrier in the central nervous system (CNS). Recently, a three-dimensional human pluripotent stem cell (hPSC)-derived ChP organoid model has been developed. This model generates cystic structures that are filled with a fluid resembling CSF and are surrounded by an epithelial layer expressing ependymal choroid plexus-specific markers. Here we describe a method to generate these choroid plexus organoids using a commercial kit and methods to extract the CSF-like fluid for use in downstream analysis.</p>