AUTHOR=Bekri Abdelhamid , Liao Meijiang , Drapeau Pierre 

TITLE=Glycine Regulates Neural Stem Cell Proliferation During Development via Lnx1-Dependent Notch Signaling

JOURNAL=Frontiers in Molecular Neuroscience

VOLUME=12

YEAR=2019

URL=https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2019.00044

DOI=10.3389/fnmol.2019.00044

ISSN=1662-5099

ABSTRACT=<p>During development of the zebrafish embryo, glycine signaling promotes the differentiation of neural stem cells (NSCs). We found that glycine signaling suppresses the expression of Ligand of Numb X1 (<italic>lnx1</italic>, Ligand of numb protein-x1), a gene of unknown function during NSC differentiation that is selectively expressed in the embryonic central nervous system (CNS). As a consequence, Numb levels were stabilized and Notch activity (measured as <italic>her4.1</italic> expression) was reduced, promoting NSC differentiation. These consequent actions were blocked by knockdown of <italic>lnx1</italic>. In contrast, <italic>lnx1</italic> overexpression increased NSC proliferation and led to defects of neural tube closure at the early stages of development. Thus, our data provide evidence that glycine/<italic>lnx1</italic> signaling modulates NSC proliferation by regulation of Notch signaling.</p>