AUTHOR=Torres Rodrigo F. , Hidalgo Cecilia , Kerr Bredford 

TITLE=Mecp2 Mediates Experience-Dependent Transcriptional Upregulation of Ryanodine Receptor Type-3

JOURNAL=Frontiers in Molecular Neuroscience

VOLUME=10

YEAR=2017

URL=https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2017.00188

DOI=10.3389/fnmol.2017.00188

ISSN=1662-5099

ABSTRACT=<p>Mecp2 is a DNA methylation reader that plays a critical role in experience-dependent plasticity. Increasing evidence supports a role for epigenetic modifications in activity-induced gene expression. Hence, candidate genes related to such phenomena are of great interest. Ryanodine receptors are intracellular calcium channels that contribute to hippocampal synaptic plasticity, dendritic spine remodeling, and participate in learning and memory processes. Here we exposed mice to the enriched environment (EE) paradigm, which through increased stimulation induces experience dependent-plasticity, to explore a role for methyl-cytosines, and Mecp2 in directing Ryanodine receptor 3 (<italic>Ryr3</italic>) transcriptional activity. EE induced a hippocampal-specific increase in the methylation of discrete cytosines located at a <italic>Ryr3</italic> isoform promoter; chromatin immunoprecipitation experiments revealed that EE increased Mecp2 binding to this <italic>Ryr3</italic> isoform promoter. Interestingly, the experimental paradigm induced robust <italic>Ryr3</italic> upregulation, accompanied by <italic>miR132</italic>-dependent suppression of <italic>p250GAP</italic>, a pathway driving synaptogenesis. In contrast to WT mice, <italic>Mecp2-null</italic> mice showed diminished levels of <italic>Ryr3</italic> and displayed impaired EE-induced <italic>Ryr3</italic> upregulation, compromising <italic>miR132</italic> dependent suppression of <italic>p250GAP</italic> and experience-dependent structural plasticity. Based on these results, we propose that Mecp2 acts as a transcriptional activator of <italic>Ryr3</italic>, contributing to experience-dependent plasticity.</p>