AUTHOR=Dierssen Mara , Fedrizzi Laura , Gomez-Villafuertes Rosa , Martinez de Lagran Maria , Gutierrez-Adan Alfonso , Sahun Ignaci , Pintado Belen , Oliveros Juan C., Dopazo Xose M., Gonzalez Paz , Brini Marisa , Mellstrom Britt , Carafoli Ernesto , NARANJO JOSE R.

TITLE=Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice

JOURNAL=Frontiers in Molecular Neuroscience

VOLUME=5

YEAR=2012

URL=https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2012.00058

DOI=10.3389/fnmol.2012.00058

ISSN=1662-5099

ABSTRACT=<p>Downstream regulatory element antagonist modulator (DREAM) is a Ca<sup>2+</sup>-binding protein that binds DNA and represses transcription in a Ca<sup>2+</sup>-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca<sup>2+</sup> homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca<sup>2+</sup>-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function.</p>