ORIGINAL RESEARCH article

Front. Mol. Biosci.

Sec. Molecular Diagnostics and Therapeutics

Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1573294

This article is part of the Research TopicThe Role of Calcium Channels in Human Health and Disease Volume IIIView all 8 articles

Role of Ca 2+ /calmodulin and PI3K/AKT signaling pathways and active ingredients of BaoTaiYin in treatment of recurrent miscarriage

Provisionally accepted
Ji  LiJi Li1Anqi  DengAnqi Deng2Huiying  ChenHuiying Chen2Shuangyan  GuoShuangyan Guo2Pingyu  WangPingyu Wang2Ruiyi  ZhangRuiyi Zhang2Wenyang  ChenWenyang Chen3Taotao  FanTaotao Fan2Lijuan  JiangLijuan Jiang1*Bing  ShenBing Shen4*
  • 1Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China
  • 2Anhui Medical University, Hefei, Anhui Province, China
  • 3Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China
  • 4Macau University of Science and Technology, Taipa, Macao, SAR China

The final, formatted version of the article will be published soon.

Introduction: BaoTaiyin (BTY) is a traditional Chinese medicine decoction. It has been used to treat recurrent miscarriage (RM). However, there are no comprehensive systematic studies to identify the chemical compositions of BTY and molecular mechanisms on RM. Finding the chemical components of BTY and clarifying the underlying processes in the treatment of RM were the goals of the study. Methods: We used ultra-high-performance liquid chromatography coupled with triple quadruple timeof-flight tandem mass spectrometry to analyze the chemical components of BTY, network analysis to predict the pharmacological effects of the identified active ingredients, and cell experiments to identify potential molecular mechanisms. Results: We found 12 active ingredients among 61 components identified in BTY. These identified activities were linked to regulatory effects on 127 key signaling pathways, targeting 107 proteins. Through network analysis, we determined that insulin-like growth factor 1 receptor, matrix metalloproteinases, PI3K, and STAT3 may be the core targets of BTY's therapeutic effects on RM. We further explored this mechanism to find that aqueous extracts of BTY significantly enhanced IGFBP2 and CaMKK2 expression and trophoblast proliferation, whereas inhibitors of IGF1R/PI3K/AKT pathway or CaMKK2 blocked the effect of BTY on trophoblast proliferation. In addition, IGFBP2 siRNA suppressed BTY-induced CaMKK2 expression. Caffeic acid, as one of components of BTY, increased intracellular Ca 2+ concentration and proliferation in trophoblast. Conclusions: Our research showed that BTY may have therapeutic benefits on RM through multiple targets and pathways, such as the IGF1R/PI3K/AKT and Ca 2+ /calmodulin signaling pathways.

Keywords: recurrent miscarriage, BaoTaiYin, Trophoblast proliferation, Ca 2+ signal, Mass Spectrometry, proliferation

Received: 08 Feb 2025; Accepted: 10 Mar 2025.

Copyright: © 2025 Li, Deng, Chen, Guo, Wang, Zhang, Chen, Fan, Jiang and Shen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Lijuan Jiang, Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China
Bing Shen, Macau University of Science and Technology, Taipa, Macao, SAR China

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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