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ORIGINAL RESEARCH article
Front. Mol. Biosci.
Sec. Genome Organization and Dynamics
Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1562357
Chromatin structure and gene transcription of recombinant p53 adenovirus vector within host
Provisionally accepted- Southern University of Science and Technology, Shenzhen, China
The recombinant human p53 adenovirus (Ad-p53) offers a promising approach for cancer therapy, yet its chromatin structure and effects on host chromatin organization and gene expression are not fully understood. In this study, we employed in situ ChIA-PET to investigate the colorectal cancer cell line HCT116 with p53 knockout, comparing them to cells infected with the adenovirus-vector expressing p53. We examined alterations in chromatin interactions and gene expression following treatment with the anti-cancer drug 5-fluorouracil (5-FU). Our results indicate that Ad-p53 forms a specific chromatin architecture within the vector and mainly interacts with repressive or inactive regions of host chromatin, without significantly affecting the expression of associated genes. Ad-p53 does not affect topologically associating domains (TADs) or A/B compartments in the host genome. These findings suggest that Ad-p53 boosts p53 expression, enhancing drug sensitivity without substantially altering host HCT116 chromatin architecture.
Keywords: Ad-p53, ChIA-PET, p53, Colon Cancer, Chromatin interaction, 3D genome
Received: 17 Jan 2025; Accepted: 17 Feb 2025.
Copyright: © 2025 Duo, Deng and Tian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Simon Zhongyuan Tian, Southern University of Science and Technology, Shenzhen, China
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