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ORIGINAL RESEARCH article

Front. Mol. Biosci.

Sec. Cellular Biochemistry

Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1551773

Colpomenia sinuosa extract mitigates lead acetate-induced testicular dysfunctions in male rats

Provisionally accepted
Layla Almutairi Layla Almutairi 1Amal Abu-Almakarem Amal Abu-Almakarem 2Noorah Saleh Al-Sowayan Noorah Saleh Al-Sowayan 3Sahar Abdulrahman Alkhodair Sahar Abdulrahman Alkhodair 4Hayat M Albishi Hayat M Albishi 4Thamir Eid Thamir Eid 4Fahad Alshanbari Fahad Alshanbari 5Najlaa Yousef Abuzinadah Najlaa Yousef Abuzinadah 6Maysa Mobasher Maysa Mobasher 7Karim El-Said Karim El-Said 8*
  • 1 Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
  • 2 Department of Basic Sciences, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi Arabia
  • 3 Department of Biology, College of Science and Arts at Unaizah, Qassim University, Buraidah, Saudi Arabia
  • 4 Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah, Makkah, Saudi Arabia
  • 5 Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, buraydah, Saudi Arabia
  • 6 Department of Biological Sciences, College of Science, Jeddah University, Jeddah, Saudi Arabia
  • 7 Department of Pathology, College of Medicine, Jouf University, Sakakah, Saudi Arabia
  • 8 Tanta University, Tanta, Egypt

The final, formatted version of the article will be published soon.

    Background: Colpomenia sinuosa brown algae contains pharmacologically active compounds with a wide spectrum of bioactivities; however, few studies have been conducted in the Mediterranean to assess their effects against heavy metal toxicity. One common nonbiodegradable contaminant that poses a serious risk to human health and the environment is lead (Pb). This study investigated the efficacy of C. sinuosa extract (CSE) treatment on testicular injury caused by lead acetate (PbAc) in rats.The phytochemical, and GC/MS profiling, and metal chelation ability of CSE were evaluated. Molecular docking studies were performed using AutoDock Vina. The oral LD50 of CSE was determined by probit analysis. 40 male rats were used as follows: Gp1 as a negative control; Gp2 was treated with 1/10 of CSE LD50 (340 mg/kg b. wt.); Gp3 was administered PbAc solution (100 mg/kg b. wt.); Gp4 was orally administered PbAc as in Gp3 and CSE as in Gp2.All treatments were given daily by gastric tube for 30 days. Body weight changes, biochemical, molecular, and histopathological examinations were investigated.The results demonstrate that CSE exerted a pronounced metal-chelating activity in vitro and contain promising phytochemicals. The LD50 of CSE was 3400 mg/kg b. wt. PbActreated rats reported significant testicular dysfunction with impaired semen analysis, biochemical, molecular, and histological changes. CSE treatment showed significant palliative effects on these dysfunctions via improvements in antioxidant status, anti-inflammatory properties, and histopathological alterations. Interestingly, CSE treatment modulates the JAK2/STAT3, and NLRP3/Caspase-1 pathways axis in PbAc-injured rats.This study for the first time investigated the biochemical and molecular mechanisms regarding the effects of CSE treatment on PbAc-induced testicular damage in male rats. CSE showed potential attenuative effect on the testis injury induced by PbAc treatment by targeting JAK2/STAT3, and NLRP3/Caspase-1 pathways. These findings suggest that CSE could be used against the adverse effect of PbAc on male repro-toxicity.

    Keywords: Colpomenia sinuosa, Brown algae, antioxidant, anti-inflammatory, Lead, testicular dysfunctions

    Received: 26 Dec 2024; Accepted: 28 Mar 2025.

    Copyright: © 2025 Almutairi, Abu-Almakarem, Al-Sowayan, Alkhodair, Albishi, Eid, Alshanbari, Abuzinadah, Mobasher and El-Said. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Karim El-Said, Tanta University, Tanta, Egypt

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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