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REVIEW article
Front. Mol. Biosci.
Sec. Cellular Biochemistry
Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1538806
This article is part of the Research Topic Exploring the Cytoskeletal Regulation of Cellular Metabolism and Disease Progression View all articles
Role of the Cytoskeleton in Cellular Reprogramming: Effects of Biophysical and Biochemical Factors
Provisionally accepted- Pirogov Russian National Research Medical University, Moscow, Russia
The cytoskeleton plays a crucial role in regulating cellular behavior, acting as both a structural framework and a mediator of mechanical and biochemical signals that influence cell fate. In the context of cellular reprogramming, modifications to the cytoskeleton can have profound effects on lineage commitment and differentiation efficiency. This review explores the impact of mechanical forces such as substrate stiffness, topography, extracellular fluid viscosity, and cell seeding density on cytoskeletal organization and mechanotransduction pathways, including Rho/ROCK and YAP/TAZ signaling. Additionally, we examine the influence of biochemical agents that modulate cytoskeletal dynamics, such as actin and microtubule polymerization inhibitors, and their effects on stem cell differentiation. By understanding how cytoskeletal remodeling governs cellular identity, this review highlights potential strategies for improving reprogramming efficiency and directing cell fate by manipulating mechanical and biochemical cues.
Keywords: Cytoskeleton, cellular reprogramming, cell fate, Mechanotransduction, Chromatin, Signal pathways, Regenerative Medicine
Received: 03 Dec 2024; Accepted: 19 Feb 2025.
Copyright: © 2025 Momotyuk, Ebrahim, Shakirova and Dashinimaev. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Erdem Dashinimaev, Pirogov Russian National Research Medical University, Moscow, Russia
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