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ORIGINAL RESEARCH article

Front. Mol. Biosci.
Sec. Molecular Diagnostics and Therapeutics
Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1492729
This article is part of the Research Topic Advancements and Future Challenges in Molecular Diagnostics and Therapeutics View all articles

Deciphering the Role of miRNA-mRNA Interactions in Cerebral Vasospasm Post Intracranial Hemorrhage

Provisionally accepted
Xiang Chu Xiang Chu 1Xiyan Zhu Xiyan Zhu 2Honghao Xu Honghao Xu 3Wenbing Zhao Wenbing Zhao 2Debin Guo Debin Guo 3Xing Chen Xing Chen 4Jinze Wu Jinze Wu 4Lei Li Lei Li 5Hao Wang Hao Wang 6*Jun Fei Jun Fei 4
  • 1 Children‘s Hospital of Chongqing Medical University, Chongqing, Chongqing Municipality, China
  • 2 Department of Military Traffic Injury Prevention and Control, Daping Hospital, Army Medical University, Chongqing, China
  • 3 Department of Emergency , Daping Hospital, Army Medical University, Chongqing, China
  • 4 Army Medical University, Chongqing, China
  • 5 Department of Trauma Medical Center, Army Medical University, Chongqing, China
  • 6 Department of Neurosurgery, Army Medical University, Chongqing, China

The final, formatted version of the article will be published soon.

    Cerebral vasospasm (CVS), a serious complication following subarachnoid hemorrhage, is associated with high rates of mortality and disability. Emerging evidence suggests that abnormal miRNA and mRNA are involved in the development of CVS. This study aims to identify essential miRNA-mRNA regulatory pairs that contribute to CVS pathogenesis. We compared the differences between spasm and non-spasm groups after cerebral hemorrhage, identifying 183 differentially expressed genes (DEGs) and 19 differentially expressed miRNAs (DEMs) related to cerebral vasospasm from the GEO database. Further functional enrichment and KEGG analysis revealed that these DEGs were enriched in several terms and pathways, including the PI3K/AKT/mTOR signaling pathway, oxidative phosphorylation pathway, RNA degradation, and folate biosynthesis signaling pathway. By employing the degree scores method for each gene, we identified the top 10 genes and developed a protein-protein interaction (PPI) network. Additionally, we discovered 19 DEMs associated with CVS and integrated them with mRNA dataset analysis to construct a miRNA-mRNA network, which comprised 8 functionally differentially expressed DEMs and 6 target mRNAs. Experimental validation confirmed the significant regulatory roles of four miRNAs (Let-7a-5p, miR-24-3p, miR-29-3p, and miR-132-3p) and two mRNAs (CDK6 and SLC2A1) in the pathogenesis of CVS. In conclusion, this comprehensive study identifies pivotal miRNAs and their target mRNAs associated with CVS through an integrated bioinformatics analysis of miRNA-mRNA co-expression networks. This approach elucidates the intricate molecular mechanisms underlying CVS and uncovers potential therapeutic targets, thereby providing a valuable foundation for refining and optimizing future treatment strategies.

    Keywords: cerebral vasospasm, intracranial hemorrhage, MiRNA-mRNA regulatory network, Bioinformatics analysis, biomarkers, Pathogenesis

    Received: 07 Sep 2024; Accepted: 13 Jan 2025.

    Copyright: © 2025 Chu, Zhu, Xu, Zhao, Guo, Chen, Wu, Li, Wang and Fei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Hao Wang, Department of Neurosurgery, Army Medical University, Chongqing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.