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ORIGINAL RESEARCH article

Front. Mol. Biosci.
Sec. Cellular Biochemistry
Volume 11 - 2024 | doi: 10.3389/fmolb.2024.1523098
This article is part of the Research Topic Advances on Molecular Mechanisms Regulating Reproduction - From Gametogenesis to Fertilization View all 3 articles

Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum

Provisionally accepted
Karol Mierzejewski Karol Mierzejewski 1*Robert Stryiński Robert Stryiński 1Iwona Bogacka Iwona Bogacka 1Monika Golubska Monika Golubska 1Mónica Carrera Mónica Carrera 2Aleksandra Kurzyńska Aleksandra Kurzyńska 2
  • 1 University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • 2 Institute of Marine Research, Spanish National Research Council (CSIC), Vigo, Madrid, Spain

The final, formatted version of the article will be published soon.

    Chronic inflammation caused by Escherichia coli infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of early pregnancy through the secretion of progesterone. Understanding the intricate interplay between inflammatory processes and reproductive organ's function is crucial for the development of effective therapeutic strategies to alleviate reproductive disorders and improve fertility. Therefore, the aim of this study was to determine the in vitro effects of lipopolysaccharide (LPS) on the proteomic profile of the porcine CL in the mid-luteal phase of the estrous cycle using LC-MS/MS analysis. The CL slices were incubated in the presence of LPS for 24 hours. We identified 12 differentially regulated proteins after treatment with LPS (7 of them were upregulated, while 5 were downregulated). The analysis showed that these proteins are involved in processes such as glucose metabolism, the tricarboxylic acid cycle (TCA), detoxification processes as well as steroid biosynthesis in the CL. Moreover, we demonstrated that LPS decreases glucose levels and increases progesterone levels in the CL. The study provides new insights into LPS-induced changes in the CL and should serve as a basis for further studies to explain as LPS affect functioning of the CL.

    Keywords: Inflammation, Mid-luteal phase, pig, glucose metabolism, Progesterone, steroidogenesis

    Received: 05 Nov 2024; Accepted: 17 Dec 2024.

    Copyright: © 2024 Mierzejewski, Stryiński, Bogacka, Golubska, Carrera and Kurzyńska. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Karol Mierzejewski, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

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