This article is a correction to:
HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA
Polona Megušar1†
Ewen D. D. Calder2,3†Tina Vodopivec Seravalli1Sergeja Lebar1
Louise J. Walport2,3*
Rok Sekirnik1*- 1Sartorius BIA Separations d.o.o., Ajdovščina, Slovenia
- 2Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, United Kingdom
- 3Protein-Protein Interaction Laboratory, The Francis Crick Institute, London, United Kingdom
A Corrigendum on
HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA
by Megušar P, Calder EDD, Vodopivec Seravalli T, Lebar S, Walport LJ and Sekirnik R (2024). Front. Mol. Biosci. 11:1443917. doi: 10.3389/fmolb.2024.1443917
In the published article, there was an error. Incorrect chromatographic system supplier was named in the Methods section.
A correction has been made to Materials and methods, Purification development, Paragraph 1. This sentence previously stated:
“Chromatographic purification was performed on an HPLC system (Knauer, Berlin, DE) with four pumps and a multiwavelength UV-Vis detector (10 mm flow cell path length). Patfix software (Sartorius BIA Separations) was used for instrument control and data acquisition.”
The corrected sentence appears below:
“Chromatographic purification was performed using PATfix system (Sartorius BIA Separations, Slovenia) equipped with quaternary pump and a multiwavelength UV-Vis detector (10 mm flow cell path length). PATfix 2.0 software (Sartorius BIA Separations) was used for instrument control and data acquisition.”
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Keywords: tRNA, in vitro transcription, HPLC, chromatography, anion exchange
Citation: Megušar P, Calder EDD, Vodopivec Seravalli T, Lebar S, Walport LJ and Sekirnik R (2024) Corrigendum: HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA. Front. Mol. Biosci. 11:1507191. doi: 10.3389/fmolb.2024.1507191
Received: 07 October 2024; Accepted: 14 October 2024;
Published: 22 October 2024.
Edited and reviewed by:
Alessio Branchini, University of Ferrara, ItalyCopyright © 2024 Megušar, Calder, Vodopivec Seravalli, Lebar, Walport and Sekirnik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Louise J. Walport, l.walport@imperial.ac.uk; Rok Sekirnik, rok.sekirnik@biaseparations.com
†These authors have contributed equally to this work