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ORIGINAL RESEARCH article

Front. Mol. Biosci.
Sec. Molecular Diagnostics and Therapeutics
Volume 11 - 2024 | doi: 10.3389/fmolb.2024.1456053

Investigating the Effects of Combined Treatment of Mesalazine with Lactobacillus Casei in the Experimental Model of Ulcerative Colitis

Provisionally accepted
Hadi Esmaili Gouvarchinghaleh Hadi Esmaili Gouvarchinghaleh 1*Shabnam Bahrami Shabnam Bahrami 2*Nahid Babaei Nahid Babaei 2*Jaleh Mohajeri Borazjani Jaleh Mohajeri Borazjani 2*Mahdieh Farzanehpour Mahdieh Farzanehpour 1*
  • 1 Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • 2 Islamic Azad University, Bushehr, Bushehr, Bushehr, Iran

The final, formatted version of the article will be published soon.

    Ulcerative colitis (UC), a common gastrointestinal disorder in affluent nations, involves chronic intestinal mucosal inflammation. This research investigated the effects of combined probiotic treatment of Lactobacillus casei (L. casei) and mesalazine on disease activity index and inflammatory factors in the UC model. 20 male BALB/c mice were utilized and divided into four groups. To induce UC, all groups received 100 μl of 4% acetic acid (AA) intra-rectally. The first group received phosphate-buffered saline (PBS) (as a positive control), the second group was treated with L. casei, the third group was treated with mesalazine and, the fourth group was treated with L. casei and mesalazine. Treatment with L. Casei and mesalazine commenced after the manifestation of symptoms resulting from UC induction. Finally, the mice were euthanized and the disease activity index, myeloperoxidase activity, nitric oxide rate, cytokines level (IL-1β, IL-6, TNF-α) and, gene expression (iNOS, COX-2, and cytokines) were evaluated.The combined treatment of L. casei and mesalazine led to a significant decrease in the levels of NO, MPO and inflammatory cytokines. In addition, the expression of cytokines, iNOS and COX-2 genes decreased in mice treated with the combination.This study shows that combined treatment of L. casei and mesalazine improves of experimental UC, which can be attributed to the anti-inflammatory properties of L. casei and mesalazine. In conclusion, this combination therapy can be considered a suitable option for the management of UC.

    Keywords: inflammatory cytokine, Inflammation, Lactobacillus casei, Mesalazine, probiotic, ulcerative colitis

    Received: 04 Jul 2024; Accepted: 13 Sep 2024.

    Copyright: © 2024 Esmaili Gouvarchinghaleh, Bahrami, Babaei, Mohajeri Borazjani and Farzanehpour. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Hadi Esmaili Gouvarchinghaleh, Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
    Shabnam Bahrami, Islamic Azad University, Bushehr, Bushehr, Bushehr, Iran
    Nahid Babaei, Islamic Azad University, Bushehr, Bushehr, Bushehr, Iran
    Jaleh Mohajeri Borazjani, Islamic Azad University, Bushehr, Bushehr, Bushehr, Iran
    Mahdieh Farzanehpour, Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.