AUTHOR=Xie Shangwei , Jiang Lihua , Song Weihua , Zheng Jialang , Liu Yifan , Chen Shun , Yan Xiaojun 

TITLE=Skeletal muscle feature of different populations in large yellow croaker (Larimichthys crocea): from an epigenetic point of view

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2024.1403861

DOI=10.3389/fmolb.2024.1403861

ISSN=2296-889X

ABSTRACT=<p>Fish skeletal muscle is composed of well-defined fiber types. In order to identify potential candidate genes affecting muscle growth and development under epigenetic regulation. Bisulfite sequencing was utilized to analyze and compare the muscle DNA methylation profiles of <italic>Larimichthys crocea</italic> inhabiting different environments. The results revealed that DNA methylation in <italic>L. crocea</italic> was predominantly CG methylation, with 2,396 differentially methylated regions (DMRs) identified through comparisons among different populations. The largest difference in methylation was observed between the ZhouShan and JinMen wild populations, suggesting that <italic>L. crocea</italic> may have undergone selection and domestication. Additionally, GO and KEGG enrichment analysis of differentially methylated genes (DMGs) revealed 626 enriched GO functional categories, including various muscle-related genes such as <italic>myh10, myf5, myf6, ndufv1, klhl31, map3k4, syn2b, sostdc1a, bag4, and hsp90ab</italic>. However, significant enrichment in KEGG pathways was observed only in the JinMen and XiangShan populations of <italic>L. crocea</italic>. Therefore, this study provides a theoretical foundation for a better understanding of the epigenetic regulation of skeletal muscle growth and development in <italic>L. crocea</italic> under different environmental conditions.</p>