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ORIGINAL RESEARCH article

Front. Mol. Biosci.
Sec. Protein Biochemistry for Basic and Applied Sciences
Volume 11 - 2024 | doi: 10.3389/fmolb.2024.1401970

Comprehensive Proteomic Characterization of Urethral Stricture Disease in the Chinese Population

Provisionally accepted
Chen Ding Chen Ding 1,2,3*Jiangtao Gao Jiangtao Gao 4Hui Liu Hui Liu 1,2Lingling Li Lingling Li 1Chunmei Guo Chunmei Guo 1Zhiyong Wang Zhiyong Wang 4Mengya Cheng Mengya Cheng 4Chao Feng Chao Feng 5Jijing Shi Jijing Shi 4Lu Chen Lu Chen 4Hui Wu Hui Wu 4Guoying Yu Guoying Yu 2
  • 1 Fudan University, Shanghai, China
  • 2 Henan Normal University, Xinxiang, Henan Province, China
  • 3 Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi, Xinjiang, China
  • 4 Zhengzhou First People's Hospital, Zhengzhou, Henan Province, China
  • 5 Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, Shanghai Municipality, China

The final, formatted version of the article will be published soon.

    Background Male urethral stricture disease (USD) is predominantly characterized by scar formation.There is still lack of effective therapeutic drugs, and comprehensive molecular characterizations of USD formation are undefined yet.Methods Proteomic profiling of twelve scar tissues and five matched normal adjacent tissues (NATs).Proteomic analysis methods were applied to explore the molecular characterizations of USD formation, including uncovering mechanistic pathways and providing novel biomarkers for scar formation.Comparative proteomic analysis showed that the extracellular matrix (ECM) and complement cascade signaling were predominant in scar tissues. COL11A1 and CD248 significantly contributed to the accumulation of ECM components. Our study presented diverse molecular mechanism of scar formation across different ages, and suggested the potential effects of PXK in the Age 1 (< 45) patients.Furthermore, immune infiltration studies indicated the therapeutic potential of inhibiting the complement system (C4A, C4B) in the Age 2 (≥ 45) patients, providing potential strategy for USD in the clinic.This study illustrated the pathogenesis of USD formation, and the diverse characteristics of USD patients with different ages, enhancing our understanding of the disease's pathogenesis and providing a valuable resource for USD.

    Keywords: Proteomics, USD, Scar formation, Immune infiltration, biomarkers

    Received: 16 Mar 2024; Accepted: 26 Jun 2024.

    Copyright: © 2024 Ding, Gao, Liu, Li, Guo, Wang, Cheng, Feng, Shi, Chen, Wu and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Chen Ding, Fudan University, Shanghai, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.