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ORIGINAL RESEARCH article

Front. Mol. Biosci.
Sec. Genome Organization and Dynamics
Volume 11 - 2024 | doi: 10.3389/fmolb.2024.1373754
This article is part of the Research Topic Exploring Aquacultural Animals and Plants by Sequencing and Re-Sequencing View all 4 articles

Analysis of genetic diversity among three Triplophysa tenuis populations by the RAD-seq

Provisionally accepted
Wenqiong Wu Wenqiong Wu 1*Junqiang Qiu Junqiang Qiu 1*Yue Lin Yue Lin 1*Xike Li Xike Li 1*Wenjuan Li Wenjuan Li 1Yuanliang Duan Yuanliang Duan 2*Yuanshuai Fu Yuanshuai Fu 1*
  • 1 Shanghai Ocean University, Shanghai, Shanghai Municipality, China
  • 2 Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan Province, China

The final, formatted version of the article will be published soon.

    To investigate the genetic diversity of Triplophysa tenuis in the Shule River Basin of Gansu Province, three populations were sequenced using RAD-seq technology. Twenty-nine microsatellite (SSR) markers with polymorphisms were finally screened to access the genetic diversity among the populations, of which 15 had high polymorphisms. Quantity of the alleles detected in the three populations of T. tenuis varied from 2 to 24. The locus with the most alleles was SSRC1, which had 24 alleles. Among the 29 SSRs, the range of effective allele number, observed heterozygosity, expected heterozygosity, and polymorphic information content were 1.246 -16.615, 0.222 -1, 0.198 -0.940, and 0.178 -0.937, respectively. Most of the identified loci were in Hardy-Weinberg equilibrium. Analysis of population structure revealed that the Yumen and Changma populations shared the same origin while the Qiaowan population was different from them. The developed SSR markers discovered in this study will contribute to the conservation research of Triplophysa tenuis and the fishery resources conservation of the Shule River, providing scientific guidance for the development and utilization of Triplophysa tenuis resources and environmental protection.

    Keywords: Triplophysa tenuis, SSR, RAD-seq, genetic diversity, population structure

    Received: 20 Jan 2024; Accepted: 19 Jun 2024.

    Copyright: © 2024 Wu, Qiu, Lin, Li, Li, Duan and Fu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wenqiong Wu, Shanghai Ocean University, Shanghai, 130012, Shanghai Municipality, China
    Junqiang Qiu, Shanghai Ocean University, Shanghai, 130012, Shanghai Municipality, China
    Yue Lin, Shanghai Ocean University, Shanghai, 130012, Shanghai Municipality, China
    Xike Li, Shanghai Ocean University, Shanghai, 130012, Shanghai Municipality, China
    Yuanliang Duan, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan Province, China
    Yuanshuai Fu, Shanghai Ocean University, Shanghai, 130012, Shanghai Municipality, China

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