AUTHOR=Dongiovanni P. , Meroni M. , Aiello Gilda , D’Amato A. , Cenzato N. , Casati S. , Damiani G. , Fenoglio C. , Galimberti D. , Grossi E. , Prati D. , Lamorte G. , Bianco C. , Valenti L. , Soggiu A. , Zapperi S. , La Porta C. A. M. , Del Fabbro M. , Tartaglia G. M. 

TITLE=Salivary proteomic profile of young healthy subjects

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2023.1327233

DOI=10.3389/fmolb.2023.1327233

ISSN=2296-889X

ABSTRACT=<p><bold>Background:</bold> The incidence of noncommunicable diseases (NCDs) has been rapidly ramped up worldwide. Hence, there is an urgent need to non-invasively detect NCDs possibly by exploiting saliva as a ‘liquid biopsy’ to identify biomarkers of the health status. Since, the absence of standardized procedures of collection/analysis and the lack of normal ranges makes the use of saliva still tricky, our purpose was to outline a salivary proteomic profile which features healthy individuals.</p><p><bold>Methods:</bold> We collected saliva samples from 19 young blood donors as reference population and the proteomic profile was investigated through mass-spectrometry.</p><p><bold>Results:</bold> We identified 1,004 proteins of whose 243 proteins were shared by all subjects. By applying a data clustering approach, we found a set of six most representative proteins across all subjects including Coronin-1A, F-actin-capping protein subunit alpha, Immunoglobulin J chain, Prosaposin, 78 kDa glucose-regulated protein and Heat shock 70 kDa protein 1A and 1B.</p><p><bold>Conclusion:</bold> All of these proteins are involved in immune system activation, cellular stress responses, proliferation, and invasion thus suggesting their use as biomarkers in patients with NCDs.</p>