AUTHOR=Nyong-Bassey Etubonesi E. , Hicks Andrew L. , Bergin Poppy , Tuite Eimer M. , Kozhevnikov Valery , Veuger Stephany 

TITLE=Effect of cyclic substituents on the anti-cancer activity and DNA interaction of ruthenium(II) bis-phenanthroline dipyridoquinoline

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2023.1252285

DOI=10.3389/fmolb.2023.1252285

ISSN=2296-889X

ABSTRACT=<p><bold>Introduction:</bold> Ruthenium(II) complexes have emerged recently as candidates for anti-cancer therapy, where activity is related to lipohilicity, cellular localization, and specific interactions with biomolecules.</p><p><bold>Methods:</bold> In this work, two novel complexes were synthesized and are reported based on the [Ru(phen)<sub>2</sub>(dipyrido[3,2-<italic>f</italic>:2′,3′-<italic>h</italic>]quinoxaline]<sup>2+</sup> framework.</p><p><bold>Results:</bold> Compared to the parent complex, annealing of cyclopenteno and cyclohexeno rings to the extended ligand substantially increased cytotoxicity towards a number of cancer cell lines, and induced apoptosis. The complexes localize in the nuclei of cancer cells and co-locate with DAPI on DNA. DNA binding studies show that both complexes bind strongly to DNA and one complex intercalates DNA like the parent, whilst the other appears to have multiple modes of interaction.</p><p><bold>Discussion:</bold> It is likely that the increased lipophilicity of the novel complexes is a key factor for increasing their cytotoxicity, rather than their DNA binding mode.</p>