AUTHOR=Aouida Mustapha , Saifaldeen Maryam , Al-Ansari Dana E. , Taleb Sara , Hssain Ali Ait , Ramotar Dindial TITLE=A CRISPR-based approach using dead Cas9-sgRNA to detect SARS-CoV-2 JOURNAL=Frontiers in Molecular Biosciences VOLUME=10 YEAR=2023 URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2023.1201347 DOI=10.3389/fmolb.2023.1201347 ISSN=2296-889X ABSTRACT=
Rapid, highly specific, and robust diagnostic kits to detect viruses and pathogens are needed to control disease spread and transmission globally. Of the many different methods proposed to diagnose COVID-19 infection, CRISPR-based detection of nucleic acids tests are among the most prominent. Here, we describe a new way of using CRISPR/Cas systems as a rapid and highly specific tool to detect the SARS-CoV-2 virus using the