AUTHOR=de Sousa Diana M. Bessa , Poupardin Rodolphe , Villeda Saul A. , Schroer Adam B. , Fröhlich Thomas , Frey Vanessa , Staffen Wolfgang , Mrowetz Heike , Altendorfer Barbara , Unger Michael S. , Iglseder Bernhard , Paulweber Bernhard , Trinka Eugen , Cadamuro Janne , Drerup Martin , Schallmoser Katharina , Aigner Ludwig , Kniewallner Kathrin M. 

TITLE=The platelet transcriptome and proteome in Alzheimer’s disease and aging: an exploratory cross-sectional study

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2023.1196083

DOI=10.3389/fmolb.2023.1196083

ISSN=2296-889X

ABSTRACT=<p><bold>Introduction:</bold> Alzheimer’s disease (AD) and aging are associated with platelet hyperactivity. However, the mechanisms underlying abnormal platelet function in AD and aging are yet poorly understood.</p><p><bold>Methods:</bold> To explore the molecular profile of AD and aged platelets, we investigated platelet activation (i.e., CD62P expression), proteome and transcriptome in AD patients, non-demented elderly, and young individuals as controls.</p><p><bold>Results:</bold> AD, aged and young individuals showed similar levels of platelet activation based on CD62P expression. However, AD and aged individuals had a proteomic signature suggestive of increased platelet activation compared with young controls. Transcriptomic profiling suggested the dysregulation of proteolytic machinery involved in regulating platelet function, particularly the ubiquitin-proteasome system in AD and autophagy in aging. The functional implication of these transcriptomic alterations remains unclear and requires further investigation.</p><p><bold>Discussion:</bold> Our data strengthen the evidence of enhanced platelet activation in aging and provide a first glimpse of the platelet transcriptomic changes occurring in AD.</p>