AUTHOR=He Xiaoyun , Di Ran , Guo Xiaofei , Cao Xiaohan , Zhou Mei , Li Xiaoyu , Xia Qing , Wang Xiangyu , Zhang Jinlong , Zhang Xiaosheng , Liu Qiuyue , Chu Mingxing 

TITLE=Transcriptomic Changes of Photoperiodic Response in the Hypothalamus Were Identified in Ovariectomized and Estradiol-Treated Sheep

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2022.848144

DOI=10.3389/fmolb.2022.848144

ISSN=2296-889X

ABSTRACT=<p>Accurate timing of seasonal changes is an essential ability for an animal’s survival, and the change in the photoperiod is the key factor affecting reproductive seasonality in mammals. Emerging evidence has suggested that multiple hypothalamic genes participate in the photoperiod-induced regulation of reproductive activities in sheep, but the mechanism is still unclear. In this study, we initially examined the plasma level of two major reproductive hormones, namely, follicle-stimulating hormone (FSH) and prolactin (PRL), under different photoperiods in ovariectomized and estradiol-treated (OVX + E<sub>2</sub>) sheep using radioimmunoassay (RIA). Of the two hormones, the concentration of PRL significantly increased with the extension of the photoperiod, while FSH showed the opposite trend. Subsequently, an examination of the transcriptomic variation between the short photoperiod (SP) and long photoperiod (LP) was conducted. Differential expression analyses and functional annotation showed that several key genes in the insulin secretion (<italic>VAMP2</italic>, <italic>PRKACB</italic>, <italic>PRKCG</italic>, and <italic>PLCB1</italic>), GnRH (<italic>MAPK13</italic>, <italic>CGA</italic>, <italic>CDC42</italic>, <italic>ATF4</italic>, and <italic>LHB</italic>) pathways, and circadian entrainment (<italic>KCNJ5</italic>, <italic>PER1</italic>, <italic>GNB2</italic>, <italic>MTNR1A</italic>, and <italic>RASD1</italic>), as well as numerous lncRNAs, including XR_173257.3, XR_173415.3, XR_001435315.1, XR_001024596.2, and XR_001023464.2, were shown potentially vital for the hypothalamic photoperiodic response. Four of the differentially expressed mRNAs and lncRNAs were validated by qPCR. The constructed mRNA–mRNA interaction networks further revealed that transcripts potentially participated in hypothalamic thyroid hormone synthesis, endocrine resistance, and neuroactive ligand–receptor interactions. The interactome analysis of lncRNAs and their targets implied that XR_173257.3 and its target arylalkylamine N-acetyltransferase (<italic>AANAT</italic>) and XR_173415.3 and its target <italic>TH</italic> might participate in the regulation of seasonal reproduction. Together, the changes in reproductive hormones and transcriptome will help to determine the important photoperiod-induced lncRNAs and mRNAs and provide a valuable resource for further research on reproductive seasonality in sheep.</p>