AUTHOR=Fitzgerald Christopher C. J. , Hedman Rikard , Uduwela Dimanthi R. , Paszerbovics Bettina , Carroll Adam J. , Neeman Teresa , Cawley Adam , Brooker Lance , McLeod Malcolm D. TITLE=Profiling Urinary Sulfate Metabolites With Mass Spectrometry JOURNAL=Frontiers in Molecular Biosciences VOLUME=Volume 9 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2022.829511 DOI=10.3389/fmolb.2022.829511 ISSN=2296-889X ABSTRACT=
The study of urinary phase II sulfate metabolites is central to understanding the role and fate of endogenous and exogenous compounds in biological systems. This study describes a new workflow for the untargeted metabolic profiling of sulfated metabolites in a urine matrix. Analysis was performed using ultra-high-performance liquid chromatography-high resolution tandem mass spectrometry (UHPLC-HRMS/MS) with data dependent acquisition (DDA) coupled to an automated script-based data processing pipeline and differential metabolite level analysis. Sulfates were identified through