AUTHOR=Zhong Lin-Kun , Deng Xing-Yan , Shen Fei , Cai Wen-Song , Feng Jian-Hua , Gan Xiao-Xiong , Jiang Shan , Liu Chi-Zhuai , Zhang Ming-Guang , Deng Jiang-Wei , Zheng Bing-Xing , Xie Xiao-Zhang , Ning Li-Qing , Huang Hui , Chen Shan-Shan , Miao Jian-Hang , Xu Bo TITLE=Identification of a 3-Gene Prognostic Index for Papillary Thyroid Carcinoma JOURNAL=Frontiers in Molecular Biosciences VOLUME=9 YEAR=2022 URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2022.807931 DOI=10.3389/fmolb.2022.807931 ISSN=2296-889X ABSTRACT=

The accurate determination of the risk of cancer recurrence is a critical unmet need in managing thyroid cancer (TC). Although numerous studies have successfully demonstrated the use of high throughput molecular diagnostics in TC prediction, it has not been successfully applied in routine clinical use, particularly in Chinese patients. In our study, we objective to screen for characteristic genes specific to PTC and establish an accurate model for diagnosis and prognostic evaluation of PTC. We screen the differentially expressed genes by Python 3.6 in The Cancer Genome Atlas (TCGA) database. We discovered a three-gene signature Gap junction protein beta 4 (GJB4), Ripply transcriptional repressor 3 (RIPPLY3), and Adrenoceptor alpha 1B (ADRA1B) that had a statistically significant difference. Then we used Gene Expression Omnibus (GEO) database to establish a diagnostic and prognostic model to verify the three-gene signature. For experimental validation, immunohistochemistry in tissue microarrays showed that thyroid samples’ proteins expressed by this three-gene are differentially expressed. Our protocol discovered a robust three-gene signature that can distinguish prognosis, which will have daily clinical application.