AUTHOR=Sakanaka Akito , Katakami Naoto , Furuno Masahiro , Nishizawa Hitoshi , Omori Kazuo , Taya Naohiro , Ishikawa Asuka , Mayumi Shota , Inoue Moe , Tanaka Isomura Emiko , Amano Atsuo , Shimomura Iichiro , Fukusaki Eiichiro , Kuboniwa Masae TITLE=Salivary metabolic signatures of carotid atherosclerosis in patients with type 2 diabetes hospitalized for treatment JOURNAL=Frontiers in Molecular Biosciences VOLUME=9 YEAR=2022 URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2022.1074285 DOI=10.3389/fmolb.2022.1074285 ISSN=2296-889X ABSTRACT=

Atherosclerosis is a life-threatening disease associated with morbidity and mortality in patients with type 2 diabetes (T2D). This study aimed to characterize a salivary signature of atherosclerosis based on evaluation of carotid intima-media thickness (IMT) to develop a non-invasive predictive tool for diagnosis and disease follow-up. Metabolites in saliva and plasma samples collected at admission and after treatment from 25 T2D patients hospitalized for 2 weeks to undergo medical treatment for diabetes were comprehensively profiled using metabolomic profiling with gas chromatography-mass spectrometry. Orthogonal partial least squares analysis, used to explore the relationships of IMT with clinical markers and plasma and salivary metabolites, showed that the top predictors for IMT included salivary allantoin and 1,5-anhydroglucitol (1,5-AG) at both the baseline examination at admission and after treatment. Furthermore, though treatment induced alterations in salivary levels of allantoin and 1,5-AG, it did not modify the association between IMT and these metabolites (pinteraction > 0.05), and models with these metabolites combined yielded satisfactory diagnostic accuracy for the high IMT group even after treatment (area under curve = 0.819). Collectively, this salivary metabolite combination may be useful for non-invasive identification of T2D patients with a higher atherosclerotic burden in clinical settings.