AUTHOR=Harada Kenji , Sakamoto Naoya 

TITLE=Cancer organoid applications to investigate chemotherapy resistance

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2022.1067207

DOI=10.3389/fmolb.2022.1067207

ISSN=2296-889X

ABSTRACT=<p>In clinical practice, a large proportion of cancer patients receive chemotherapy, yet tumors persist or acquire resistance; removing this obstacle could help to lower the number of cancer-related fatalities. All areas of cancer research are increasingly using organoid technology, a culture technique that simulates the <italic>in vivo</italic> environment <italic>in vitro</italic>, especially in the quickly developing fields of anticancer drug resistance, drug-tolerant persisters, and drug screening. This review provides an overview of organoid technology, the use of organoids in the field of anticancer drug resistance research, their relevance to clinical information and clinical trials, and approaches to automation and high throughput.</p>