AUTHOR=Shang Haitao , Chen Yichi , Wang Chunyue , Zhang Shentao , Wu Bolin , Liang Xitian , Liu Zhao , Wang Qiucheng , Cheng Wen TITLE=RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC JOURNAL=Frontiers in Molecular Biosciences VOLUME=8 YEAR=2022 URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.791331 DOI=10.3389/fmolb.2021.791331 ISSN=2296-889X ABSTRACT=

Sonodynamic therapy is widely used in the treatment and research of hepatocellular carcinoma. A novel targeted nanobubble complex mediated with Hematoporphyrin monomethyl ether and Lonidamine was structured as a sensitizer, characterized the properties, and studied the therapeutic effect on hepatocellular carcinoma. The complexes can promote the apoptosis of hepatocellular carcinoma cells and work better in combination with sonodynamic therapy. The differential expression of multiple types of RNA in hepatocellular carcinoma with sonodynamic therapy can be identified accurately with high-throughput RNA sequencing. The differential expressions of mRNA, lncRNA, and circRNA were analyzed by RNA-Seq. The enrichment analyses (Gene Ontology and KEGG) prompted the meaningful genes and pathways in the process of sonodynamic therapy in hepatocellular carcinoma cells. HMME-LND@C3F8-NBs conjugated with ultrasound is confirmed efficiently for inhibiting the development of hepatocellular carcinoma cells, and it is a combination of multiple genes and mechanisms.