AUTHOR=Ganesan Raja , Vasantha-Srinivasan Prabhakaran , Sadhasivam Deepa Rani , Subramanian Raghunandhakumar , Vimalraj Selvaraj , Suk Ki Tae 

TITLE=Carbon Nanotubes Induce Metabolomic Profile Disturbances in Zebrafish: NMR-Based Metabolomics Platform

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.688827

DOI=10.3389/fmolb.2021.688827

ISSN=2296-889X

ABSTRACT=<p>The present study aims to investigate the metabolic effects of single-walled carbon nanotubes (SWCNT) on zebrafish (<italic>Danio rerio</italic>) using <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy. However, there is no significant information available regarding the characterization of organic molecules, and metabolites with SWCNT exposure. Noninvasive biofluid methods have improved our understanding of SWCNT metabolism in zebrafish in recent years. Here, we used targeted metabolomics to quantify a set of metabolites within biological systems. SWCNT at various concentrations was given to zebrafish, and the metabolites were extracted using two immiscible solvent systems, methanol and chloroform. Metabolomics profiling was used in association with univariate and multivariate data analysis to determine metabolomic phenotyping. The metabolites, malate, oxalacetate, phenylaniline, taurine, sn-glycero-3-phosphate, glycine, N-acetyl mate, lactate, ATP, AMP, valine, pyruvate, ADP, serine, niacinamide are significantly impacted. The metabolism of amino acids, energy and nucleotides are influenced by SWCNT which might indicate a disturbance in metabolic reaction networks. In conclusion, using high-throughput analytical methods, we provide a perspective of metabolic impacts and the underlying associated metabolic pathways.</p>