AUTHOR=Yang Yi , Feng Qingxing , Hu Kun , Cheng Feng 

TITLE=Using CRISPRa and CRISPRi Technologies to Study the Biological Functions of ITGB5, TIMP1, and TMEM176B in Prostate Cancer Cells

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.676021

DOI=10.3389/fmolb.2021.676021

ISSN=2296-889X

ABSTRACT=<p>Although ITGB5, TIMP1, and TMEM176B are abnormally expressed in several cancers, their molecular biological mechanisms in prostate cancer cells are still to be investigated. The gene regulation technogies based on CRISPR transcription factors could be used to investigate the biological functions of genes in cancer. In this study, we used CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) technologies to regulate the transcription of ITGB5, TIMP1, and TMEM176B in prostate cancer cells. Through a series of cellualr experiments, we found that inhibition of ITGB5 or activation of TIMP1 and TMEM176B suppress prostate cancer. The three genes synergistically affect the proliferation, invasion and migration capabilities of cancer cells.</p>