AUTHOR=Li Qi , Chai Lijuan , Dong Gaopan , Zhang Xiaomeng , Du Lupei 

TITLE=NBD-Based Environment-Sensitive Fluorescent Probes for the Human Ether-a-Go-Go–Related Gene Potassium Channel

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.666605

DOI=10.3389/fmolb.2021.666605

ISSN=2296-889X

ABSTRACT=<p>Three environment-sensitive probes were developed for the hERG channel based on the nitrobenzoxadiazole fluorophore herein. After careful evaluation, probes <bold>M1</bold> and <bold>M3</bold> were found to have a high affinity for imaging the hERG channel in the cell-based experiment. Compared with other fluorescent labeling technologies (such as fluorescent proteins), these probes afford a convenient and economical method to determine hERG channel <italic>in vitro</italic> and <italic>in cellulo</italic>. Therefore, these probes are expected to be applicable for usage in physiological and pathological studies of hERG channels and have the potential to establish a screening system for hERG channels.</p>