AUTHOR=Sui Yutong , Liu Jiayin , Zhang Jing , Zheng Zena , Wang Ziwei , Jia Zhenghu , Meng Ziyu 

TITLE=Expression and Gene Regulation Network of Adenosine Receptor A2B in Lung Adenocarcinoma: A Potential Diagnostic and Prognostic Biomarker

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.663011

DOI=10.3389/fmolb.2021.663011

ISSN=2296-889X

ABSTRACT=<p><italic>Adenosine</italic><italic>receptor A2B</italic> (<italic>ADORA2B</italic>) encodes a protein belonging to the G protein–coupled receptor superfamily. Abnormal expression of <italic>ADORA2B</italic> may play a pathophysiological role in some human cancers. We investigated whether ADORA2B is a potential diagnostic and prognostic biomarker for lung adenocarcinoma (LUAD). The expression, various mutations, copy number variations, mRNA expression levels, and related network signaling pathways of <italic>ADORA2B</italic> were analyzed using bioinformatics-related websites, including Oncomine, UALCAN, cBioPortal, GeneMANIA, LinkedOmics, KM Plotter, and TIMER. We found that <italic>ADORA2B</italic> was overexpressed and amplified in LUAD, and a high <italic>ADORA2B</italic> expression predicted a poor prognosis for LUAD patients. Pathway analyses of <italic>ADORA2B</italic> in LUAD revealed <italic>ADORA2B</italic>-correlated signaling pathways, and the expression level of <italic>ADORA2B</italic> was associated with immune cell infiltration. Furthermore, <italic>ADORA2B</italic> mRNA and protein levels were significantly higher in human LUAD cell lines (A549 cells and NCl-H1299 cells) than in normal human bronchial epithelial (HBE) cells, and the transcript levels of genes positively or negatively correlated with <italic>ADORA2B</italic> were consistent and statistically significant. siRNA transfection experiments and functional experiments further confirmed these results. <italic>In vitro</italic> results were also consistent with those of bioinformatics analysis. Our findings provide a foundation for studying the role of <italic>ADORA2B</italic> in tumorigenesis and support the development of new drug targets for LUAD.</p>