AUTHOR=Bücker Robert , Hogan-Lamarre Pascal , Miller R. J. Dwayne 

TITLE=Serial Electron Diffraction Data Processing With diffractem and CrystFEL

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2021.624264

DOI=10.3389/fmolb.2021.624264

ISSN=2296-889X

ABSTRACT=<p>Serial electron diffraction (SerialED) is an emerging technique, which applies the snapshot data-collection mode of serial X-ray crystallography to three-dimensional electron diffraction (3D Electron Diffraction), forgoing the conventional rotation method. Similarly to serial X-ray crystallography, this approach leads to almost complete absence of radiation damage effects even for the most sensitive samples, and allows for a high level of automation. However, SerialED also necessitates new techniques of data processing, which combine existing pipelines for rotation electron diffraction and serial X-ray crystallography with some more particular solutions for challenges arising in SerialED specifically. Here, we introduce our analysis pipeline for SerialED data, and its implementation using the <italic>CrystFEL</italic> and <italic>diffractem</italic> program packages. Detailed examples are provided in extensive supplementary code.</p>