AUTHOR=Joshi Bishnu , Singh Bhupender , Nadeem Aftab , Askarian Fatemeh , Wai Sun Nyunt , Johannessen Mona , Hegstad Kristin 

TITLE=Transcriptome Profiling of Staphylococcus aureus Associated Extracellular Vesicles Reveals Presence of Small RNA-Cargo

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=7

YEAR=2021

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2020.566207

DOI=10.3389/fmolb.2020.566207

ISSN=2296-889X

ABSTRACT=<p>Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen <italic>Staphylococcus aureus</italic> has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by <italic>S. aureus</italic> MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction.</p>