AUTHOR=Gardner Andrew F. , Jackson Kiserian M. , Boyle Madeleine M. , Buss Jackson A. , Potapov Vladimir , Gehring Alexandra M. , Zatopek Kelly M. , Corrêa Jr. Ivan R. , Ong Jennifer L. , Jack William E. 

TITLE=Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=6

YEAR=2019

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2019.00028

DOI=10.3389/fmolb.2019.00028

ISSN=2296-889X

ABSTRACT=<p>A variant of 9°N DNA polymerase [Genbank ID (<ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAA88769.1" xmlns:xlink="http://www.w3.org/1999/xlink">AAA88769.1</ext-link>)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.</p>