AUTHOR=Navarro Paula P. , Stahlberg Henning , Castaño-Díez Daniel TITLE=Protocols for Subtomogram Averaging of Membrane Proteins in the Dynamo Software Package JOURNAL=Frontiers in Molecular Biosciences VOLUME=5 YEAR=2018 URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2018.00082 DOI=10.3389/fmolb.2018.00082 ISSN=2296-889X ABSTRACT=
Cryo-electron tomography allows low-resolution three-dimensional (3D) viewing of cellular organelles and macromolecular complexes present as multiple copies within a tomogram. These structures are computationally extracted and averaged in order to obtain high-resolution 3D structures, and provide a map of their spatial distribution and interaction with their biological microenvironment. To do so, we apply the user-friendly