AUTHOR=Sen Partho , Kemppainen Esko , Orešič Matej TITLE=Perspectives on Systems Modeling of Human Peripheral Blood Mononuclear Cells JOURNAL=Frontiers in Molecular Biosciences VOLUME=4 YEAR=2018 URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2017.00096 DOI=10.3389/fmolb.2017.00096 ISSN=2296-889X ABSTRACT=

Human peripheral blood mononuclear cells (PBMCs) are the key drivers of the immune responses. These cells undergo activation, proliferation and differentiation into various subsets. During these processes they initiate metabolic reprogramming, which is coordinated by specific gene and protein activities. PBMCs as a model system have been widely used to study metabolic and autoimmune diseases. Herein we review various omics and systems-based approaches such as transcriptomics, epigenomics, proteomics, and metabolomics as applied to PBMCs, particularly T helper subsets, that unveiled disease markers and the underlying mechanisms. We also discuss and emphasize several aspects of T cell metabolic modeling in healthy and disease states using genome-scale metabolic models.