AUTHOR=Delaforge Elise , Milles Sigrid , Huang Jie-rong , Bouvier Denis , Jensen Malene Ringkjøbing , Sattler Michael , Hart Darren J. , Blackledge Martin 

TITLE=Investigating the Role of Large-Scale Domain Dynamics in Protein-Protein Interactions

JOURNAL=Frontiers in Molecular Biosciences

VOLUME=3

YEAR=2016

URL=https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2016.00054

DOI=10.3389/fmolb.2016.00054

ISSN=2296-889X

ABSTRACT=<p>Intrinsically disordered linkers provide multi-domain proteins with degrees of conformational freedom that are often essential for function. These highly dynamic assemblies represent a significant fraction of all proteomes, and deciphering the physical basis of their interactions represents a considerable challenge. Here we describe the difficulties associated with mapping the large-scale domain dynamics and describe two recent examples where solution state methods, in particular NMR spectroscopy, are used to investigate conformational exchange on very different timescales.</p>