AUTHOR=Ortiz-Olvera Nayeli , Fernández-Figueroa Edith A. , Argueta-Donohué Jesús , Miranda-Ortíz Haydee , Ruiz-García Erika 

TITLE=Case Report: Oral and fecal microbiota in a super-donor: the healthy microbiota paradigm for fecal transplantation

JOURNAL=Frontiers in Microbiomes

VOLUME=2

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiomes/articles/10.3389/frmbi.2023.1219960

DOI=10.3389/frmbi.2023.1219960

ISSN=2813-4338

ABSTRACT=<p>Despite the numerous fecal microbiota transplantation trials that have been carried out, knowledge about the actual composition of a “healthy microbiota” remains scarce. The aim of this research was to describe the differences in the composition of oral and fecal microbiotas in a super-donor. The microbiota analysis was done using next-generation sequencing of the V3 and V4 regions of the bacterial 16S rRNA gene. The biodiversity found in the mouth was very rich, with 56 species identified, and there was a predominance of the genera <italic>Veillonella</italic>, <italic>Haemophilus</italic>, and <italic>Streptococcus</italic>. It is worth mentioning the presence (2.33%) of <italic>Fusobacterium nucleatum</italic> in the mouth. In feces, the genera <italic>Bacteroides</italic> and <italic>Faecalibacterium</italic> predominated, with the species <italic>F. prausnitzii</italic> being the most abundant. This analysis shows that the diversity of the microbiota of a super-donor plays a fundamental role in the effectiveness of its product in fecal matter transplantation. This suggests that transplanted gut microorganisms have the ability to maintain or recover health in a dynamic process between the microbiota and the host. Our findings encourage further research which will result in the development of bacterial therapies in infectious and inflammatory diseases.</p>