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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Extreme Microbiology
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1571458
Discovery of Iodinin-like compounds by Co-Culture of Amycolatopsis luridaTRM64739 and Bacillus haynesii
Provisionally accepted- College of Life Sciences and Technology, Tarim University, Alar, China
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Actinomycetes are a significant source of natural products. Amycolatopsis, a rare actinomycete, is particularly noted for its robust potential in secondary metabolite production, but most of the biosynthetic gene clusters (BGCs) are silent. This study employed genome mining and co-culture techniques to explore the secondary metabolites produced by Amycolatopsis lurida TRM64739. A novel BGC was identified in A.lurida TRM64739 using antiSMASH and phylogenetic analysis, this new BGC was activated through co-culturing A. lurida TRM64739 with Bacillus haynesii. Subsequently, a series of phenazine compounds were isolated and identified as compound 1(1,6-Dimethoxyphenazine), compound 2(1,6-Dihydroxyphenazine), compound 3(phenazine-1-carboxylic acid), compound 4(6-hydroxy-1-methoxyphenazine), and compound 5(1,6p-chlorophenylphenazine) using 1 D NMR, 2 D NMR, and MS techniques. Among these, compounds 1-4 are known, while compound 5(1,6-p-chlorophenylphenazine) represents a new compound and has exhibited antimicrobial activity to clinically drug-resistant strains (A. baumannii ATCC19606, P. aeruginosa ATCC27853) and plant pathogenic bacteria (E. amylovora ATCC BAA-2158). Our work also demonstrates that the combined approach of genome mining and activation of silent BGCs is a useful method for the discovery of new natural products.
Keywords: genome mining, co-culture, Amcolatopsis lurida TRM64739, Bacillus haynesii, Phenazine compounds
Received: 05 Feb 2025; Accepted: 09 Apr 2025.
Copyright: © 2025 Liu, Luo, Xia, Wan and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Li-Li Zhang, College of Life Sciences and Technology, Tarim University, Alar, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.