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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbe and Virus Interactions with Plants
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1561057
This article is part of the Research Topic Bacillus and Pseudomonas as Plant Friends: Molecular, Physiological and Ecological Interactions View all 8 articles
Bacillus vallismortis acts against ginseng root rot by modifying the composition and microecological functions of ginseng root endophytes
Provisionally accepted
Yang Hu 1 Meng-Yuan Lei 1 Yi-Xin Yang 1
Zhuo Sun 1* Wan Wang 2*
Zhongming Han 1
Lin Cheng 1 Ze-Liang Lv 1 Mei Han 1 Li-Min Yang 1- 1 Jilin Agriculture University, Changchun, China
- 2 Changchun Medical College, Changchun, Jilin Province, China
The endophytic microbiome serves a crucial function as a secondary line of defense against pathogen invasion in plants. This study aimed to clarify the mechanism of action of the ginseng plant growth-promoting rhizobacteria (PGPR) Bacillus vallismortis SZ-4 synergizing with endophytic microorganisms in the prevention and control of root rot. Ginseng root samples from a susceptible group (CK) with a disease level of 0-2 and a biocontrol group (BIO) treated with strain SZ-4 were collected We employed high-throughput sequencing to examine the microbial community structure of ginseng roots at different disease levels, explore beneficial endophytic bacteria, and evaluate the efficacy of strain SZ-4 in mitigating root rot through synergistic interactions with ginseng endophytic flora. The application of the PGPR B. vallismortis SZ-4 biocontrol fungicide has been found to help ginseng resist Fusarium solani by modulating the richness and structure of endophytic microbial populations. The endophytic bacteria HY-43 and HY-46 isolated from ginseng roots treated with B. vallismortis SZ-4 were identified as Bacillus velezensis based on morphological, physiological, and biochemical characteristics, as well as 16S rDNA and gyrB sequencing analyses. The endophytic bacteria HY-43 and HY-46 were combined with strain SZ-4 to generate the bacterial consortia CS4-43 and CS4-46, respectively. Both CS4-43 and CS4-46 significantly enhanced the inhibitory effects of the single strain SZ-4, as well as HY-43 and HY-46, against ginseng root rot, while also promoting plant growth. These findings offers a theoretical foundation for studying the microecological prevention and control of ginseng diseases as well as new insights for conducting research on the efficient and precise management of plant diseases.
Keywords: ginseng, root rot, Bacillus vallismortis, Bacillus velezensis, biocontrol strain, Endophytic flora, community structure
Received: 15 Jan 2025; Accepted: 21 Mar 2025.
Copyright: © 2025 Hu, Lei, Yang, Sun, Wang, Han, Cheng, Lv, Han and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Zhuo Sun, Jilin Agriculture University, Changchun, China
Wan Wang, Changchun Medical College, Changchun, Jilin Province, China
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