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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microorganisms in Vertebrate Digestive Systems
Volume 16 - 2025 | doi: 10.3389/fmicb.2025.1560887
This article is part of the Research Topic The Interaction Between Food Ingredients and Gut Microbiome on Health and Disease View all 11 articles
Bacteroides uniformis Alleviates Fatty Liver Hemorrhagic Syndrome in Dawu Golden Phoenix Laying Hens through Gut Microbiota and Arachidonic Acid Metabolism
Provisionally accepted- 1 Hebei Agricultural University, Baoding, Hebei, China
- 2 Hebei Jinkun Animal Pharmaceutical Co. LTD, Xinji, China
- 3 Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Hainan, China
This study explored the impact of Bacteroides uniformis (B. uniformis) on fatty liver hemorrhagic syndrome (FLHS) induced by a high-energy and low-protein (HELP) diet in laying hens, mainly focusing on the hepatic lipid metabolism, the gut microbiota and arachidonic acid (AA) metabolism. A total of 120 (210-day-old) Dawu Golden Phoenix laying hens were randomly divided into four groups: control group (CON) were fed a standard diet and given a daily gavage of PBS, while the other groups were all fed with a HELP diet to induce FLHS and given a daily gavage of PBS (MOD), 1 × 10 9 CFU/mL B. uniformis (BUL), and 1 × 10 11 CFU/mL B. uniformis (BUH) for 70 days, respectively. All hens were given 1 mL daily by gavage. Each group had 6 replications with 5 hens per replication. The results showed that B. uniformis increased egg production rate and feed conversion ratio and decreased body weight, liver index, and abdominal fat rate (P < 0.05). B. uniformis treatment reduced the liver lipid accumulation through reducing the levels of Triglyceride (TG), Total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), alanine transaminases (ALT), aspartate transaminases (AST) in serum and significantly elevating high-density lipoprotein cholesterol (HDL-C) (P < 0.05). The results indicated that B.uniformis altered the gut microbiota. Specifically, the abundance of Bacteroides was higher and the relative abundances of Treponema, Helicobacter and Spirochaetota were lower than the MOD group (P < 0.05). Moreover, targeted metabolomic analysis showed that supplementation of B. uniformis significantly elevated 6-keto-PGF1α and AA levels, combined with significantly reduced thromboxane B2 (TXB2), leukotriene D4 (LTD4), 8-isoprostaglandin F2α (8-iso-PGF2α), 12S-hydroxyeicosatetraenoic acid (12S-HETE), 15S-hydroxyeicosatetraenoic acid (15S-HETE), 9-S-hydroxy-octadecadienoic acid (9S-HODE) and 13-S-hydroxy-octadecadienoic acid (13S-HODE) levels (P < 0.05). In conclusion, oral intake of B. uniformis could improve liver function, gut microbiota, and AA metabolism to ameliorate FLHS in Dawu Golden Phoenix laying hens.
Keywords: Fatty liver hemorrhagic syndrome, Lying hens, Bacteroides uniformis, Gut Microbiota, Arachidonic Acid
Received: 20 Jan 2025; Accepted: 25 Mar 2025.
Copyright: © 2025 Zhang, Ma, Du, He, Zhang, Ma, Liu and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hailong Liu, Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Hainan, China
Xinghua Zhao, Hebei Agricultural University, Baoding, 071001, Hebei, China
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